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SP-5020  (vector laboratories)


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  • 95

    Structured Review

    vector laboratories SP-5020
    Sp 5020, supplied by vector laboratories, used in various techniques. Bioz Stars score: 95/100, based on 227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/casein+solution/10x+Casein+Solution/pmc13080397-47-0-5
    Average 95 stars, based on 227 article reviews
    SP-5020 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Dasatinib and Quercetin Combination Increased Kidney Damage in Acute Folic Acid-Induced Experimental Nephropathy
    Article Snippet: .. Sections were incubated for 1 h at room temperature with Casein Solution (Vector Laboratories, Newark, CA, USA) to remove non-specific protein binding sites. ..

    Article Title: Dasatinib and Quercetin Combination Increased Kidney Damage in Acute Folic Acid-Induced Experimental Nephropathy
    Article Snippet: .. Sections were incubated for 1 h at room temperature with Casein Solution (Vector Laboratories, Newark, CA, USA) to remove non-specific protein binding sites. ..

    Article Title: The Role of LVV‐H7 in Alcohol‐Induced Reward Mechanisms
    Article Snippet: The adequate antibodies confirmed the presence of proteins (Biorbyt, UK). .. The blotted membranes were treated with casein solution (Vector Laboratories, USA) as a blocking agent for 45 min at room temperature, washed three times with Tris‐buffered saline (TBS; Sigma‐Aldrich), and subsequently incubated with antibody for 1 h, according to the manufacturer's recommendations (NMDAR1 orb99445, ACE1 orb107454, OPRM1 orb216172, DARPP‐32 orb106530). .. After extensive washing, the blots were incubated with the horseradish peroxidase‐conjugated goat anti‐rabbit IgG secondary antibody (65‐6120, Thermo, USA) for 1 h with TBS supplemented with 0.1% Tween 20.

    Article Title: Injury-induced KIF4A neural expression and its role in Schwann cell proliferation suggest a dual function for this kinesin in neural regeneration
    Article Snippet: Fixed samples from human nerve tissue and rat peripheral nerves, DRG and spinal cord tissues were either i) embedded in Tissue-Tek® optimal cutting temperature (OCT) cryo-compound (Sakura, Finetek, Torrance, CA, USA), sectioned into 10 μm tissue cryosections, subsequently thaw mounted and stored at –20°C until further use; or ii) dehydrated in a graded series of ethanol (70%–100%), cleared in xylol, paraffin-embedded and sectioned into 5 μm tissue sections. .. After OCT washing or paraffin washing and rehydration, cryosections were blocked with 10% donkey serum (Sigma-Aldrich, Burlington, MA, USA) and paraffin-embedded sections were incubated with antigen retrieval buffers (10 mM sodium citrate, 0.05% Tween 20, pH 6; or 1 mM EDTA, pH 8), treated with H 2 O 2 to quench endogenous peroxidase (in case of HRP-conjugated secondary antibodies) and blocked with 2.5% horse serum (Vector Laboratories, Burlingame, CA, USA) and 1× casein solution (Vector Laboratories). ..

    Article Title: CCN2 Activates Cellular Senescence Leading to Kidney Fibrosis in Folic Acid-Induced Experimental Nephropathy.
    Article Snippet: .. Sections were incubated for 1 h at room temperature with Casein Solution (Vector Laboratories, Newark, CA, USA) to remove non-specific protein binding sites. ..

    Article Title: CCN2 Activates Cellular Senescence Leading to Kidney Fibrosis in Folic Acid-Induced Experimental Nephropathy
    Article Snippet: .. Sections were incubated for 1 h at room temperature with Casein Solution (Vector Laboratories, Newark, CA, USA) to remove non-specific protein binding sites. ..

    Protein Binding:

    Article Title: Dasatinib and Quercetin Combination Increased Kidney Damage in Acute Folic Acid-Induced Experimental Nephropathy
    Article Snippet: .. Sections were incubated for 1 h at room temperature with Casein Solution (Vector Laboratories, Newark, CA, USA) to remove non-specific protein binding sites. ..

    Article Title: Dasatinib and Quercetin Combination Increased Kidney Damage in Acute Folic Acid-Induced Experimental Nephropathy
    Article Snippet: .. Sections were incubated for 1 h at room temperature with Casein Solution (Vector Laboratories, Newark, CA, USA) to remove non-specific protein binding sites. ..

    Article Title: CCN2 Activates Cellular Senescence Leading to Kidney Fibrosis in Folic Acid-Induced Experimental Nephropathy.
    Article Snippet: .. Sections were incubated for 1 h at room temperature with Casein Solution (Vector Laboratories, Newark, CA, USA) to remove non-specific protein binding sites. ..

    Article Title: CCN2 Activates Cellular Senescence Leading to Kidney Fibrosis in Folic Acid-Induced Experimental Nephropathy
    Article Snippet: .. Sections were incubated for 1 h at room temperature with Casein Solution (Vector Laboratories, Newark, CA, USA) to remove non-specific protein binding sites. ..

    Blocking Assay:

    Article Title: Activation and maturation of antigen-specific B cells in nonectopic lung infiltrates are independent of germinal center reactions in the draining lymph node
    Article Snippet: .. After peroxidase inactivation and blocking with casein solution (Vector Laboratories, Burlingame, CA, USA), the sections were stained with the antibodies listed in Supplementary Table . .. Tyramide signal amplification was performed via peroxidase-coupled anti-FITC or anti-digoxigenin antibodies and Alexa Fluor 488-, 555-, or 647-coupled tyramide (Thermo Fisher Scientific).

    Article Title: The Role of LVV‐H7 in Alcohol‐Induced Reward Mechanisms
    Article Snippet: The adequate antibodies confirmed the presence of proteins (Biorbyt, UK). .. The blotted membranes were treated with casein solution (Vector Laboratories, USA) as a blocking agent for 45 min at room temperature, washed three times with Tris‐buffered saline (TBS; Sigma‐Aldrich), and subsequently incubated with antibody for 1 h, according to the manufacturer's recommendations (NMDAR1 orb99445, ACE1 orb107454, OPRM1 orb216172, DARPP‐32 orb106530). .. After extensive washing, the blots were incubated with the horseradish peroxidase‐conjugated goat anti‐rabbit IgG secondary antibody (65‐6120, Thermo, USA) for 1 h with TBS supplemented with 0.1% Tween 20.

    Article Title: Activation and maturation of antigen-specific B cells in nonectopic lung infiltrates are independent of germinal center reactions in the draining lymph node.
    Article Snippet: .. After peroxidase inactivation and blocking with casein solution (Vector Laboratories, Burlingame, CA, USA), the sections were stained with the antibodies listed in Supplementary Table S2. .. Tyramide signal amplification was performed via peroxidase-coupled anti-FITC or anti-digoxigenin antibodies and Alexa Fluor 488-, 555-, or 647-coupled tyramide (Thermo Fisher Scientific).

    Staining:

    Article Title: Activation and maturation of antigen-specific B cells in nonectopic lung infiltrates are independent of germinal center reactions in the draining lymph node
    Article Snippet: .. After peroxidase inactivation and blocking with casein solution (Vector Laboratories, Burlingame, CA, USA), the sections were stained with the antibodies listed in Supplementary Table . .. Tyramide signal amplification was performed via peroxidase-coupled anti-FITC or anti-digoxigenin antibodies and Alexa Fluor 488-, 555-, or 647-coupled tyramide (Thermo Fisher Scientific).

    Article Title: Activation and maturation of antigen-specific B cells in nonectopic lung infiltrates are independent of germinal center reactions in the draining lymph node.
    Article Snippet: .. After peroxidase inactivation and blocking with casein solution (Vector Laboratories, Burlingame, CA, USA), the sections were stained with the antibodies listed in Supplementary Table S2. .. Tyramide signal amplification was performed via peroxidase-coupled anti-FITC or anti-digoxigenin antibodies and Alexa Fluor 488-, 555-, or 647-coupled tyramide (Thermo Fisher Scientific).

    Saline:

    Article Title: The Role of LVV‐H7 in Alcohol‐Induced Reward Mechanisms
    Article Snippet: The adequate antibodies confirmed the presence of proteins (Biorbyt, UK). .. The blotted membranes were treated with casein solution (Vector Laboratories, USA) as a blocking agent for 45 min at room temperature, washed three times with Tris‐buffered saline (TBS; Sigma‐Aldrich), and subsequently incubated with antibody for 1 h, according to the manufacturer's recommendations (NMDAR1 orb99445, ACE1 orb107454, OPRM1 orb216172, DARPP‐32 orb106530). .. After extensive washing, the blots were incubated with the horseradish peroxidase‐conjugated goat anti‐rabbit IgG secondary antibody (65‐6120, Thermo, USA) for 1 h with TBS supplemented with 0.1% Tween 20.



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